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Quantitative polymerase chain reaction (qPCR) was performed on a QuantStudio 3 instrument using Fast Advanced Master Mix Reagents (Fisher Scientific) and a concentration of 1 ng cDNA per reaction

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The resulting ARGs provide a full representation of the historical genealogical relationships among all individuals in our three sampled populations, offering greater insight beyond the scope of a single consensus tree, thus capturing complex patterns of genomic inheritance

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