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Quantitative real-time PCR Total RNA was extracted from primary neurons isolated from E16/17 as described previously (Table 1

Yet, its energy consumption is among the lowest on this list ($75 per year), only bested by the smart Mila ($58)

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This is hardly surprising given the preemptive mourning Depp does in his account of Ryder, anticipating and engraving loss into his relationship at the very pinnacle of their love

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