Common Causes of Peptide Degradation Many different processes and factors can affect peptides: Peptide hydrolysis caused by moisture exposure Oxidation resulting from oxygen and exposure to light Changes in temperature Bacterial degradation in peptide solutions Contamination during laboratory handling Repeated freeze-thaw cycles (Sigma-Aldrich, 2023a
Inspect solution before each use
The strength for TCA used in TCA Cross technique varies - but we generally use between 50-100% is what has been used and studied clinically
Heavey MK, Hazelton A, Wang Y, Garner M, Anselmo AC, Arthur JC, Nguyen J
The structure shows that the asymmetric unit contains one molecule of OsDHAR and adopted a canonical GST fold, consisting of three -strands and three -helices in the N-terminal subdomain (residues 484) and six -helices in the C-terminal subdomain (residues 94210), although OsDHAR showed modest sequence identity (~20%) with GSTs (Fig
Some peptides tolerate short-term refrigeration at 28C before reconstitution, but freezer storage is optimal