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The cycle threshold (CT) values of gene expression were normalized to the housekeeping gene -actin, and the expression ratios were calculated using the CT method
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2018;4:133 Blumberg HM, Burman WJ, Chaisson RE, Daley CL, Etkind SC, Friedman LN, et al
[DOI] [PubMed] [Google Scholar] Yamazaki M, Matsuoka T, Yasui K, Komiyama A, Akabane T (1989) Dopamine inhibition of superoxide anion production by polymorphonuclear leukocytes
Statistical analysis was performed for three independent biological replicates, with the final differential expression between strains calculated using the package DESeq2 (Love et al , 2014)
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