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GLP-1R localisation and GLP-1R-induced PKA signalling at ERMCSs in primary mouse and human islets To validate the interaction of GLP-1R with VAPB and SPHKAP at ERMCSs in a physiologically relevant setting with endogenous levels of receptor expression, proximity ligation assays (PLAs) were performed in purified mouse primary islets using one of the few existing validated mouse GLP-1R antibodies from the Developmental Studies Hybridoma Bank 51,52,53 , with GLP-1RVAPB and GLP-1RSPHKAP interactions readily observed in exendin-4-stimulated islets (Fig

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