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Transgenic expression of Glud1 (glutamate dehydrogenase 1) in neurons: in vivo model of enhanced glutamate release, altered synaptic plasticity, and selective neuronal vulnerability

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After PBS washing, HRP Polymer Quanto (TL-125-QPH, Thermo Fisher Scientific, USA) was applied, and immunoreactivity was visualized with DAB chromogen (TA-125-HA, Thermo Fisher Scientific, USA), followed by hematoxylin counterstaining, in accordance with previously described standard immunohistochemical procedures.79 Immunohistochemical expression was evaluated using the H-score method, in which staining intensity (weak = 1, moderate = 2, strong = 3) and the percentage of positive cells were assessed in three random fields.80,81 The H- score was calculated as H-score = Pi (i + 1), where i denotes staining intensity and Pi the percentage of cells at each intensity

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Alzheimer disease: An update on pathobiology and treatment strategies