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The His 6 -tagged RAGRagulator complex was then added to the reactions and incubated on ice for 30 min

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In ADP-ribosylation factor 2-interacting protein (Arfip2)-knockout human podocytes and Arfip2 knockout and streptozotocin (STZ)-induced mouse models, Arfip2 deficiency worsens autophagy in diabetic mice and leads to loss of pedicle protrusions, histopathological alterations, and early albuminuria, and in human podocytes, Arfip2 deficiency interferes with autophagy-associated protein 9a (ATG9A) transport and the PINK1/Parkin pathway, resulting in impaired mitochondrial fission and reduced mitophagy, thus Arfip2 may be a novel factor regulating autophagy and mitochondrial homeostasis in foot cells [111]

Hiramoto et al