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Statistical analysis Normal distribution was assessed by Shapiro-Wilk test and histograms

Identification and validation of enriched biological pathways To obtain a comprehensive understanding of the influence of smoking on cancer from an epigenetic perspective, we conducted stepwise pathway-based analyses for the two types of samples using the bioinformatics tools of Ingenuity Pathway Analysis (IPA) 48 , EnrichNet 49 , and Genetrail 50