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[8][9] Teens who use nicotine are more likely to develop attention deficits and may be at increased risk for later psychiatric conditions, including anxiety and depression

This desensitization effect reduces the inhibitory effects of GABA leading to excitability of the dopamine reward system ( Materials and Methods E-CIG E-liquid mixtures of 50% glycerin, 50% propylene glycol, and 24 mg/ml nicotine (purchased from Xtreme Vaping) were prepared in our laboratory as described in our recent study ( via application of negative pressure, 2L/ min for 1 s, by the InExpose system (SciReq), and followed by continuous negative pressure of 1L/min for 3 additional seconds