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The amplification was performed using Illumina Nextera XT adapter-ligated eubacterial V3 region-specific primers, 341F and 534R, with five different base modifications (Wang and Qian, 2009

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Results Over a median follow-up period of 12.96 years, 9054 and 10,301 participants were diagnosed with COPD based on in utero tobacco exposure and age of smoking initiation, respectively