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Statistical analysis GraphPad Prism 8.2.1 software (GraphPad Software Inc., San Diego, CA, USA) was used for statistical analysis and data processing

The GLP-1 receptor pathway involves GLP-1 binding to its receptor (GLP-1R), which activates G-proteins, leading to increased cyclic adenosine monophosphate (cAMP) levels and subsequent activation of protein kinase A (PKA)

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EPA is known for its anti-inflammatory properties, which can help protect the brain against age-related damage and maintain cognitive function