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Nicotine and Tobacco Research 2014;16(6):8159 [cited 2014 Oct 31]

Environ Res 216:114717

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Enzyme-linked immunosorbent assay (ELISA) To confirm hepatic levels of pro-inflammatory cytokines, such as tumor necrosis factor alpha (TNF) and interleukin 1 beta (IL1), protein samples were prepared and measured using ELISA kits (Invitrogen, CA, United States) in accordance with the manufacturers protocol