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Nuclear magnetic resonance imaging of the leaves by UC Berkeley chemist David Wemmer will enable the scientists to spot any carbon bottlenecks in the plant and refine their metabolic engineering

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tomentosiformis 15 were also used (Supplementary Table 11)

In addition, to control for any interactions between nicotine and ETOH, the same ETOH concentrations present in the extract doses were added to the respective nicotine alone doses (e.g., 32% ETOH at 1.25 mg/kg nicotine, 24% at 1.0 mg/kg nicotine, etc.)