The figures take into account statistics on women in 1996 who smoked an average of 13.6 cigarettes per day, which would make each cigarette amount to a 20-minute loss of life expectancy over-all
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Once the cigarette is out of the pack, thats what you see
Therefore, it would need proper cleaning to get to the source of the smell
On day 14, cells were labelled with calcein-AM (C3100, Molecular Probes, New York, USA), by incubation in 1 M calcein-AM reconstituted in DMSO, for one hour at 37 C