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Enzyme-Linked Immunosorbent Assay (ELISA) Sera from mice were prepared and 500-fold serum dilutions were tested for total IgE and OVA-specific IgE to confirm the presence of allergic responses using the LBIS Mouse IgE ELISA Kit (# 633-02899, Fujifilm, Gunma, Japan) and LBIS Mouse anti-OVA-IgE ELISA Kit (# 633-07659, Fujifilm, Gunma, Japan), according to the manufacturers instructions

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