Preventing adolescents from smoking initiation is crucial in achieving this, as quitting smoking is challenging once habitualised and the probability of quitting is inversely proportional to initiation age (35, 36)
Laboratory procedures involved centrifuging serum samples at 5000 RPM for five minutes at 4 C and the serum was stored in Eppendorf tubes and maintained deeply frozen at 20 C until testing [25]
However, changes in nicotine intake, appetite, metabolism, and behaviour when you start or stop vaping can lead to weight fluctuations for some individuals
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(US Surgeon Generals Report, 1988, p
