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Then, 15 g of protein was mixed with NativePAGE sample buffer (Invitrogen), Coomassie G-250 and protease inhibitor, loaded into the lanes of a 312% Bis-Tris NativePAGE gel (Invitrogen) and run according to the manufacturers instructions

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Concentrations were then corrected for these with the following formula: where MetCorr is the corrected concentration of a metabolite, MetConcAbs is this metabolite concentration from the LCModel output and fGM, fWM and fCSF stand for, respectively, fractions of GM, WM and CSF in the voxel of interest

Its effectiveness in regulating blood sugar and aiding weight reduction has made it a popular choice for many patients