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The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article
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Endothelial NO/cGMP signaling To mimic the experimental conditions of the isometric tension studies with isolated aortas, cultured endothelial cells were incubated for 24 h with the extracts, followed by determination of L-arginine-to-L-citrulline conversion and accumulation of cGMP in response to activation of endothelial NO synthase by the Ca 2+ ionophore A23187
It is then heat-treated and put under pressure to further develop the flavor and body, and finished by vacuum-sealing in 8 oz
Please note that starting from 3.0, the amount of liquid used varies depending on the mode
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