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Hydrocarbon solvents present a less aggressive option, still providing effective cleaning while being gentler on fabrics

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For the cotinine analysis, 175 L diphenylamine, the internal standard, was added to the urine samples, then 1 mL of each urine sample was hydrolyzed with 50 L of 0.1M sodium hydroxide and extracted with 500 L of chloroform

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If you happen across one of these, eat something sweet