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Then, 1 mL of the supernatant was collected for gradient dilution up to 10 7

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The inhalation of secondhand smoke can lead to respiratory issues, such as coughing, wheezing, and exacerbated asthma symptoms, especially in children and individuals with pre-existing respiratory conditions

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1 Introduction Tobacco ( Nicotiana tabacum L.) is a global economic crop extensively cultivated in many countries (Feng et al., 2023)