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The overexpression of the Nt TPC1A fusion protein was accompanied by subtle, but specific phenotypic responses, such as impaired cell elongation (depending on auxin), reduced intracellular calcium content (rescued by supplementation of calcium to the medium), partial resistance to the calcium-channel blocker gadolinium, and a higher sensitivity to the cell-death inducing protein harpin from the phytopathogenic bacterium Erwinia amylovora

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Clipper lighters are refillable, thanks to the valve at the bottom of the lighter