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People who are present in a hotboxed room are exposed to elevated levels of marijuana

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A similar holder made of wood, meerschaum or bakelite and with an amber mouthpiece was used for cigars and was a popular accessory for men from the Edwardian period until the 1920s

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For the fungal gene, the ITS1F-ITS2R hypervariable regions were amplified using the ITS1F (5-CTTGGTCATTTAGAGGAAGTAA-3) and ITS2R (5-GCTGCGTTCTTCATCGATGC-3) primer pair