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Basolateral media was replaced with fresh PC-ALI every 48 or 72 h until harvest for wholemount IF labeling on the ALI day indicated (all KOs) or qRT-PCR, wholemount FISH, and Ussing analysis ( FOXI1 and POU2F3 KOs)

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Mechanisms of Nicotines Impact on Testosterone Nicotine affects testosterone levels through several mechanisms: Hormonal Disruption: Nicotine interferes with the hypothalamic-pituitary-gonadal (HPG) axis, which regulates testosterone production