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Sin estas condiciones, se corre el riesgo de que la calidad de las hojas sea mala y el rendimiento se reduzca

A second factor may be that the different methodologies to study calcium channels, all come with their specific constraints, leading to discrepant conclusionsgenetic ablation will give a static snapshot and is obscured by pleiotropy, localisation studies with fluorescent sensors have to deal with the problem that sensing of calcium will impact the level of free calcium, and patch-clamp studies require isolation of the membrane systems from their cellular context

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