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Upon reaching 90% confluency, cells were rinsed with Dulbeccos phosphate-buffered saline (DPBS) without calcium and magnesium then detached using 1.5 mL of 0.25% trypsin EDTA/DPBS with polyvinylpyrrolidone for 1.5 min at 37 C

doi: 10.1016/j.psychres.2011.05.037 54 WintererG

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The other stream goes through your feet up, across the torso, around the spine to the top your head

Statistics and reproducibility AT and TH performed all MR analyses independently, with replication of the same results and conclusions