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Briefly, top 8000 highly variable genes (HVGs) were detected and principal component analysis (PCA) was performed on the variable gene matrix to reduce noise, and top 50 components were used for downstream analyzes

So the effort moved slowly at first

Single-nucleotide-resolution sequencing of human N6-methyldeoxyadenosine reveals strand-asymmetric clusters associated with SSBP1 on the mitochondrial genome