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FSC/SSC, singlet, and CD4 gates were defined per sample, whereas Th1, Th17, Th22, and Treg gates were consistently applied across secondary lymphoid tissues (spleen and draining lymph nodes) and CNS samples using the same antibody panel

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The extent to which the age based sales restrictions will be ignored, or evaded by diversion and unlicensed sales (and potential harms of a parallel illegal/quasi-legal market) therefore remains to be seen