Prior to imaging, seedlings were rinsed in sterile water and stained with 500 nM propidium iodide (PI, 50 M stock solution in H 2 O) for 1 minute, then rinsed again with water to get rid of the excess staining
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Simply calculate your new total volume and divide 10mg by that number to get your new concentration
As mechanisms to dispose of both hydrogen peroxide and lipid hydroperoxides are essential to life, this indicates that in contrast to the multiple metabolic pathways that can be utilized to dispose of hydrogen peroxide, pathways for the disposal of lipid hydroperoxides are limited