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RT-qPCR RNA isolation, Primer Sequences, RT-PCR on Tfam, OPA1, Drp1, Fis1, Mfn1, Mfn2 using MyiQ single-color Real-Time thermal cycler [30] and RT-PCR for IL-6, IL-8, IL-1, Mn-SOD, PINK1, PPARGC1, OPA1 and TFAM using Taqman were performed as described in the online data Additional file 1