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A good rule is to think in layers

The peptide content remains 600mg regardless

Basically, Im a mess and at the end of my rope trying to fix everything using traditional approaches

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These findings are in parallel with an earlier study reported in the preclinical study for resveratrol, largely present in red wine, in which it has chemopreventive effects against prostate cancer [96]

Samples were then desalted again, and fractionated to 12 fractions using a 57-minute linear gradient from 3% to 32% acetonitrile (ACN) in 10 mM ammonium bicarbonate pH 8.0, at a flow rate of 0.25 mL/min, with an Agilent 1100 HPLC