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For siRNA detection, 15 g of Low-molecular-weight RNAs was separated on 15% polyacrylamide7 M urea gel and transferred to a Hybond-N + membrane in 0.5 X TBE at 0.8 mA cm 2 for 1 h

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In siblings with similar genetic susceptibility for inflammatory bowel disease, smokers tend to develop Crohns disease and non-smokers develop ulcerative colitis

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