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Enhanced Blood Cell Production: Vitamin B12 is pivotal for the production of red blood cells

Subsequently, 200 L of lysis buffer was added to the pellet, and the cells were disrupted on ice with an ultrasonic cell disruptor for 5 min

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Ultimately, pediatric obesity management should extend beyond weight reduction to address developmental safety, microbiota-endocrine interactions, and sex-specific variability, with molecular and integrative approaches paving the way for safer, more effective, and ethically sustainable protocols