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RT-PCR RNA was extracted from fully matured HBE cells with the AllPrep DNA/RNA Mini kit (80204, QIAGEN AG, Germantown, MD) and treated with DNase I (M0303S, New England BioLabs Inc., Ipswich, MA) before reverse transcription with the iScript cDNA Synthesis Kit (1708891, Bio-Rad)

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UNESCO United Nations Educational, Scientific and Cultural Organization

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And most also expect cannabis to be legal in all 50 states within the next five years