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cAMP-dependent mobilization of intracellular Ca 2+ stores by activation of ryanodine receptors in pancreatic beta-cells

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Equal amounts of total protein (25 g per sample) were mixed with 6 Laemmli sample buffer (4% SDS, 10% 2-mercaptoethanol, 20% glycerol, 0.004% bromophenol blue, and 0.125 M TrisHCl, pH 6.8), heated at 95 C for 5 min, and briefly centrifuged.Proteins were separated on 10% SDSpolyacrylamide gels using the Mini-PROTEAN Tetra Cell system (Bio-Rad, USA) at 90 V for stacking and 100150 V for resolving