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Reverse transcription followed by qPCR was performed in the same reaction using the Universal Probes One-Step PCR kit (Bio-Rad Laboratories) and the TaqMan primers: Ppib (Mm00478295_m1) , Aqp5 (Mm00437578_m1), and Gkn3 (Mm01183934_m1) (all from Thermofisher)