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In order to evaluate the effect of berberine on insulin secretion, we measured the serum insulin level at fasting

Very expensive ($500-$2,000+ per treatment course)

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Ferroptotic cells frequently demonstrate mitochondrial swelling, a diminished number of cristae, reduction of mitochondrial membrane potential, and enhanced mitochondrial membrane permeability, collectively indicative of mitochondrial dysfunction [39]

Prevents cellular damage

In research protocols, this can be either desirable (for models examining appetite regulation, weight gain, or catabolic disease states) or undesirable (for protocols where appetite stimulation is a confounder)