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Briefly, after dissection the brain tissue was incubated for 10 min at 37 C in PBS containing trypsinEDTA (0.025%/0.01% w/v) and then mechanically dissociated at room temperature with a fire-polished Pasteur pipette
Another possibility of the direct effects of GLP-1 and ghrelin in the brain has been proposed since both peptides cross the brain blood barrier 25,35
GLP-1 medications are reshaping more than healthcare theyre influencing the grocery aisle
doi:10.1016/j.ad.2011.03.003 (Epub 2011 May 4
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