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The inhalation time was set at 3 s and two different protocols were applied, according to the selected power level: (1) three puffs of 3 s with 27 s of rest between them for the 15-W and 25-W power levels, (2) two puffs of 3 s with 27 s of rest between them for the 50-W power levels (only two puffs were needed to avoid an overload of the cascade impactor stages)

Lower expression levels of miR-320d in lung tissue of non-COPD smokers and COPD patients compared to never smokers To evaluate whether miR-320d is differently expressed in COPD, its expression in whole lung tissue samples of COPD patients was compared to never smokers and (current/former) control smokers from independent screening (n=91) and validation (n=84) cohorts

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