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PubMed, Jones, Bridgette L., et al

On the food side: bitter foods like arugula, radicchio, dandelion greens trigger bitter taste receptors in the gut that stimulate GLP-1, and turmeric and ginger also have emerging evidence

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The cell lysate was clarified by centrifugation at 16,000 g and 4 C for 1 hour, and the supernatant was loaded onto the Ni-NTA resin (EMD Millipore) and washed with buffer containing 50 mM NaPi (pH 7.5), 500 mM NaCl, 520 mM imidazole, 5% (v/v) glycerol

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