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Skin color is determined by melanin, a pigment produced by cells called melanocytes

References: Tomas M, et al

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Underlying mechanisms include inhibiting TRL binding to the negatively charged cell surface where the enzyme is resident [16] and inhibiting activation of the enzyme by displacing the LPL activator apoC-II from the surface of the TRL particle [17]

Your choice of binding buffer will influence the binding capacity of the beads , so if you are capturing substantially less than 8 mg of protein per mL of resin, and your protein isnt really big, then it is worth considering if your binding buffer is limiting the capture efficiency of the beads