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16S rRNA Amplicon Analysis The raw sequence data were subjected to ambiguity filtering and quality filtration using NGSQC toolkit (Patel and Jain, 2012), and the paired-end reads were assembled using FLASH (Mago and Salzberg, 2011)

These effects usually last between 5 and 20 minutes, depending on the dose and the type of snuff tobacco used

You should let it rest there as the flavour and nicotine are released gradually

It is up to the user to check these rules, and it is the user's responsibility to comply with them

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