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To examine the possible harmful effects, we used growth media exposed to aerosolized e-liquids with and without flavors or to CSE (positive control), to treat immortalized human bronchial epithelial cells (BEAS-2B), NCI-H460 cells, and differentiated monocytic cells (THP-1)

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2 Removing these flavors from the market could save kids from a lifetime of addiction

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This mystery was partially explained when a second host factor, MPIP1, a DnaJ-like protein with potential chaperone activity, was determined to interact with TMV MP and NTH201 in a yeast-three hybrid screen (Shimizu et al., 2009)