Biochar is defined as a "carbon-rich product produced through pyrolysis, which involves heating biomass like wood, manure, or leaves in a closed container with limited air supply." Biochar has gained increased attention due to its distinctive qualities, including high carbon content, cation exchange capacity, a large specific surface area, and a stable structure
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Subsequently, the tissue samples were dehydrated by being immersed in ascending ethanol concentrations and later dipped thrice in xylene
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