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8 ml of PBS was added to the remaining solution and centrifuged at 1,000 g for 10 min at 4C

The solubility score based on the Protein-Sol web server was computed to be 0.796, 0.801, and 0.821 (cutoff: 0.45) for nGLP1-DARPin, mGLP1-DARPin-1, and mGLP1-DARPin-2, respectively

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This enhanced potency explains why semaglutide produces greater weight loss at lower relative doses

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