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The natural component T1 , isolated from Gastrodia elata , is a strong competitive inhibitor of mushroom tyrosinase (IC 50 = 0.53 M, K i = 58 6 nM), which is superior to kojic acid (IC 50 = 40.69 M) and more effective than other known natural products

Effects of caffeine administration Caffeine is the most consumed psychoactive substance, producing different effects depending on the dose/concentration, time (acute or chronic), route, and stage of life during exposure [12]

doi: 10.1155/2022/3208037 383

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The mixtures were heated to 60C and maintained at this temperature for 1, 3, 6, 18, 36, 48 and 72 h (ag) respectively