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Serious safety considerations require careful patient selection and monitoring
308 Experimentally-induced depletion or attenuation of reactive astroglia markedly exacerbates the clinical progression, spread of inflammation and tissue loss in EAE during the induction phases of the disorder, 81,309,310 whereas similarly induced depletion of proliferating reactive astrocytes at later times during the progressive phase ameliorates disease, and this amelioration is at least in part due to removal of astrocyte Ccl2 production
beta arrestin1/2 assays HEK 293A Cells were transiently transfected with GLP-1R-Rluc8 and rat -arrestin1-Venus at a ratio of 1:4 using a standard PEI protocol (6:1 ratio PEI:DNA) and seeded in to poly-D-Lysine coated white 96-well culture plates at a density of 30,000 cells/well
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Noticing this led researchers to study and approve GLP-1 analogs to treat obesity in people with or without diabetes
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