- Then, protect with an Fmoc protecting group before adding the next amino acid residue
By creatively splitting 31 amino acids of a main peptide chain of the semaglutide are into a 3-site amino acid polypeptide chain (MI chain) and a 28-site amino acid polypeptide chain (MII chain), the possibility of efficiently and quickly preparing the semaglutide under an escherichia coli preparation system is realized
- Use an acid (such as trifluoroacetic acid) or base (such as n-butylamine) to disconnect the polypeptide chain from the solid support
SUBSTITUTE SHEETS (RULE 26) b) recovering semapeptide, wherein semapeptide amino acid sequence is Glu-Gly-Thr-Phe- Thr-Ser-Asp-Val-Ser-Ser-Tyr-Leu-Glu-Gly-Gln-Ala-Ala-Lys-Glu-Phe-Ile-Ala-Trp-Leu-Val-Arg- Gly-Arg-Gly
That said, at commercial scale, recombinant production can offer a major cost advantage over chemical synthesis, which requires expensive protected amino acid building blocks and coupling reagents
The solubility of semaglutide changes with pH
