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doi:10.1371/journal.pone.0052572

Cell 140 , 871882 (2010)

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The outcomes were liver biopsy-based (NAFLD activity score [NAS], fibrosis stage, and NASH resolution), noninvasive technique-based (liver fat content on proton magnetic resonance spectroscopy [ 1 H-MRS] and controlled attenuation parameter [CAP]), biological, and anthropometric indicators

After washing in PBS, astrocytes were fixed in 4% paraformaldehyde (PFA) for 1520 min

This suggests that the experimental variables substantially impact the assay outcomes