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The mechanism involves clearance of senescent Leydig cells that secrete pro-inflammatory SASP factors (IL-1, IL-6, TGF-) which impair testosterone production in neighboring healthy cells

Emerging evidence suggests that tirzepatide may influence systemic inflammatory markers, though there is no FDA indication for anti-inflammatory therapy, and such use would be considered off-label

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Quantification of the protein was performed using the Lambert-Beer law, the absorption of the protein was measured at 280 nm and extinction coefficients were calculated by the ExPASy ProtParam tool 63

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