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Additionally, various molecular techniques, including Western blotting, reverse transcription, quantitative PCR, immunofluorescence, and immunohistochemistry, were utilized to examine the expression levels of angiogenesis-related factors and the Notch/NF-B pathways-related factors

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For morphological analysis of the vertebrae, we used a digital CMOS X-ray detector (Model 2315, Dexela, London, UK) at 45 kV and 120 mA, with a 2.5 s exposure time 32

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